LC-HR/MS is an advanced analytical technique that combines liquid chromatography with high-resolution mass spectrometry, allowing for more accurate determination of molecule mass of ions. Because ions are sorted based on their mass-to-charge ratio, even the slightest differences in mass can be detected, making this method useful for applications that require accurate mass determination.
Two different data acquisition modes in LC-HR/MS are TSIM (targeted selection ion monitoring) and Full Scan. TSIM will drop overall data points per monitored targeted mass points, and Full Scan offers data points for all masses and enables the ability to mine data post-acquisition. It’s recommended to use TSIM when you need to identify specific compounds with high sensitivity and selectivity, and Full Scan when you’re interested in identifying a wide range of compounds.
Our tenured team of scientists have extensive experience applying LC-HR/MS to identify and quantify:
- RNA therapeutics
- Peptides
- Proteins
- Lipid Nanoparticles
