Aliri colocalizes the drug and its target directly within the microenvironment, without any labeling or chemical modification of the administered molecule to confirm that they are colocalized at a concentration higher than the IC50.
Using a unique expertise of label-free Quantitative Mass Spectrometry Imaging technology and histology-based immunostaining techniques, we precisely localize and quantify the concentration of active compounds (small molecules & peptides) with the target at the cellular level by:
- Overlapping the drug and the target biodistribution images thanks to our multimodal approach and to our data science expertise.
- Scoring the percentage of drug reaching the target
- Differentiating the drug and the related metabolites
- Quantifying the drug concentration at the site of action
By integrating label-free drug quantification with high-resolution spatial analysis, it enables precise colocalization of the drug and its target, providing deeper insights into target engagement. This unparalleled precision sets a new standard for drug development to confirm the dose, formulation and administrative mode selections at preclinical and clinical stages. Aliri can also combine the target exposure evaluation with the in situ Pharmacodynamics investigation (link to the in situ PK/PD page) by measuring key markers associated with the target biological pathway.