The ability to understand tissue heterogeneity is critical to answering key biological questions that will enable you to advance your molecule to the next phase. Profiling molecular phenotypes while retaining spatial information of expression is not only suggesting new relationships among signaling molecules, but it is also deepening our understanding of disease mechanisms that are critical to find spatial signatures of biomarkers. Therefore, leveraging spatial biology is relevant to understand the effect of the investigated drug.
Aliri has developed routine analysis of innovative molecular profiling high-multiplex assays combined with image analysis and digital pathology workflow services. These services are backed by a team of industry experts, so that you can correlate histopathology to molecular content organization for many applications in oncology, CNS, or metabolic disorders.
How can Aliri help you characterize disease state?
- Provide spatially differentiated profiling of tumor driver and immuno-oncology proteins
- Discover biomarkers related to location and signaling of specific cells
- Analyze pathways across tissue structures and complexity
- Provide basis for identifying clinically relevant associations with drugs
Learn more about the equipment we use:
| Category | Matrix assisted laser desorption ionization technique (MALDI) imaging | Imaging Mass Cytometry Hyperion | RNAScope | GeoMx Nanostring Ncounter | Immuno-fluorescence | Laser ablation inductively coupled plasma (LA-ICP) imaging |
|---|---|---|---|---|---|---|
| Overview | Detect intact endogenous molecules amino acids, metabolites, small drugs from frozen of FFPE tissues | Detects protein and RNA using metal tagged antibodies from any type of tissues (Frozen of FFPE) | Detect siRNA or mRNA at single cells resolution with a very high sensitivity | Combined with NGS, or standalone, the GeoMx makes it possible to quantify the distribution of hundreds of proteins or RNA from tissue section | Detects endogenous elements from tissue section from frozen of FFPE tissues | |
| Resolution | 10μm | SubCellular | SubCellular | 10μm | SubCellular | 10μm |
| Molecule Type | Small molecules, peptides, lipids, metabolites | Large panels of biomarkers available for oncology, immuno-oncology, inflammation and fibrosis | RNA and oligonucleotides | Proteins and oligonucleotides | Various elements such as gadolinium, platinum, lithium, sodium, potassium, magnesium, calcium, iron, cobalt, nickel, copper, zinc, silver | |
| Plexing | +1000 | +40 | Up to 5 | Up to +1000 | Up to 5 | +10 |
| Sample Prep | Cryostat, Microtome, Histological AutoStainer | Cryostat, Microtome, Histological AutoStainer, Ventana Discovery Ultra | Cryostat, Microtome, Histological Autostainer, Ventana Discovery Ultra | Cryostat, Microtome, Histological AutoStainer, Ventana Discovery Ultra | Cryostat, Microtome, Histological AutoStainer, Ventana Discovery Ultra | Cryostat, Microtome, Histological Autostainer, Ventana Discovery Ultra |
| Quantification | Absolute quantification | Absolute quantification | Absolute quantification | Relative quantification | Relative quantification | Absolute quantification |
| Digital Pathology | Combined with our data scientist, we analyze all the data into our proprietary software, customized data analysis process or vendor software depending on the request | Combined with our data scientist, we analyze all the data into our proprietary software, customized data analysis process or vendor software depending on the request | Combined with our data scientist, we analyze all the data into our proprietary software, customized data analysis process or vendor software depending on the request | Combined with our data scientist, we analyze all the data into our proprietary software, customized data analysis process or vendor software depending on the request | Combined with our data scientist, we analyze all the data into our proprietary software, customized data analysis process or vendor software depending on the request | Combined with our data scientist, we analyze all the data into our proprietary software, customized data analysis process or vendor software depending on the request |
1000+
Molecular Images Analyzed
100+