Batch alignment of mass spectrometry imaging (MSI) metabolome through data integration
Mass spectrometry imaging (MSI) is a critical tool used to investigate tissues and molecules in spatial bioanalysis. Technical challenges in MSI, also known as batch-effects, have proven to impede reliable comparison of data from large-scale studies performed in translational clinical research.
Meaningful analysis of data generated in large-scale studies is critical for medicine and biology studies. This application note focuses on a batch correction method designed to minimize impedance of batch-effects and allow reliable identification of biological clusters and their comparison.
Download to learn more about batch alignment through data integration.
Automated approach to quantify single molecule in complex tissues
This application note focuses on an efficient analysis method which enables the quantification of molecules at the single-cell level. Accurate RNA quantification in single molecules is crucial to understand the dynamics of gene expression and regulation.
In this study, individual molecules were imaged in fixed cells and DNA staining was performed in tumoral areas of the tissue. Analysis results showcase quantification of single cells and provide an understanding of cell-to-cell interactions in cancer cells and other molecules.
Download to learn more about quantifying single molecules in complex tissues.
Oligonucleotides: Where we started, where we stand, and where we would like to go
Oligonucleotides, which are short chains of nucleotides that make up DNA and RNA, are used in a wide range of applications in bioanalysis and play a critical role in understanding the molecular mechanisms of living cells and diseases.
Organic Synthetic Chemist, Troy Voelker PhD, discusses common methods to support the bioanalysis of oligonucleotides (OGNTs) and a future perspective regarding areas for improvement.
His insights explores a range of analytical techniques for OGNTs, including:
-Basics of oligonucleotide bioanalysis
-Selecting an approach
-Methods of extraction
-Handling Hybridization methods
-Goals for future analysis
Discover how future predictions for oligonucleotide bioanalysis will help improve medicine, therapeutics and genetic testing.
Site overview fact sheet
As a frontrunner in the field of bioanalysis and spatial bioanalysis, we understand the importance of precision in delivering reliable data for our clients, and we take pride in the knowledge and capabilities of our teams.
With each site presenting unique offerings, instrumentation, and compound classes, we guarantee:
- Drug and lead optimization
- Target engagement
- PD efficacy
- Patient stratification
Explore our expertise, capabilities and specialized services that distinguish us from the rest.
Drug discovery and bioanalysis solutions
Due to limited time, funds, and resources within discovery, it can be challenging to strike the right balance of speed, cost, and quality. Drug discovery quick turnarounds are imperative to keep your program on track.
Our specialized discovery team provides support for small molecules, peptides & proteins, and oligonucleotides. Our ability to provide technical expertise and quick turnaround will instill confidence and enable you to move onto the next phase in your program with ease.
Trusting us with your discovery project grants you access to:
- 48-hour data delivery
- Data in the format that meets your needs
- Identification for molecule advancement
- Access to expert scientists
- Flexibility to change or accelerate timelines
Download our comprehensive guide to learn how our quick turnarounds for lead optimization can expedite your process
from discovery to clinical.
Aliri Overview brochure
The increased focus on precision medicine, oncology and other therapies has changed how companies are planning for drug discovery and clinical development. There are many challenges to navigate, including:
- Robust regulatory environment
- Developing a thorough program but also advancing with speed
- Having access to a breadth of technical expertise to assist
- And much more…
This is why choosing a partner like Aliri is critical to the success of your program. We provide a range of bioanalysis, spatial bioanalytical, and spatial biology services to support the lifecycle of your drug. Our labs have proven operational excellence across a range of traditional and innovative technologies, and the experience to support the unique needs of your development program.
Utilize our team of seasoned scientists and technical experts to help advance your molecule from discovery through clinical:
- Access quality data for filing your IND, NDA, or CTA with speed
- Anticipate technical issues or potential roadblocks that may cause delays
- Showcase drug efficacy in the pertinent spatial context up to the cellular level to improve drug candidate evaluation and selection
- Maximize the value of your drug discovery and development investments
Check out our company overview to learn more about our services and how we can work together to provide you with accurate data FAST.
RNAscope assay: Single cell transcriptomics for drug target discovery
When dealing with biomarkers in drug target discovery, profiling the tissue transcriptome in its spatial organization is key to understanding and predicting that tissue’s response to immunotherapy, because the tissue function within the body relies on the precise spatial organization of the cells. This is especially true when dealing with complex and heterogeneous tissues, such as tumors.
With tumors, the relationship between the cells and their environment is what ultimately shapes a patient’s fate. Applying the RNAscopeTM assay allows that evaluation of the presence of transcripts within a spatial context. This technique then ultimately can predict the tumor’s response to immunotherapy.
To further explain the impact of the RNAscope assay, this application note details a case study on a non-small-cell lung cancer (NSCLC) sample to predict its response to immunotherapy. This case study:
- Investigates the presence of the PD-L1 drug target transcript and the Granzyme B (GZMB) biomarker of clinical outcome transcript
- Explains quality control measures taken to validate the hybridization technique
Looks at the heterogenous distribution of transcripts in the NSCLC tissue section & examines the quantification of the PD-L` and GZMB to draw conclusions on the tissue’s reaction
Aliri – Industry-leading Services
Choosing a bioanalytical CRO to assist you with bringing your drug to market is critical to the success of your project. Aliri Bioanalysis provides a wide range of bioanalysis , spatial bioanalysis, and spatial biology solutions to support the lifecycle of your drug.
Work with us to utilize our team of experience scientists and industry experts to advance your molecule from discovery through clinical:
- Achieve quality data for filing your IND, NDA and CTA with speed
- Forecast technical issues or potential roadblocks that may cause delays
- Maximize the growth of your drug discovery and development investments
Check out how our advanced technologies can advance your molecule and bring your drug to market with ease.
Timeline planning tips for importing or exporting biological samples
Missed deadlines in drug development often stem from improper planning and coordination during the import and export of biological samples. Establishing and executing a proper plan for this part of the process can help ensure that your program stays on-track, while avoiding unnecessary stress such as costly delays, lost shipments, etc.
Download this infographic to learn more about this robust process:
- Permits and government agency requirements
- Human vs animal requirements
- Timelines and expectations
- Resources and process planning
QMSI vs. QWBA: Evaluating Benefits and Risks
Knowing when to use Quantitative Mass Spectrometry Imaging (QMSI) instead of Quantitative Whole-Body Autoradiography (QWBA) can make or break a timeline and budget for pharma and biotech companies.
Consider the following key advantages when selecting which technique is right for your drug development program:
QSMI Advantages:
- Label-free quantification of thousands of analytes
- Excellent differentiation among parent drugs, metabolites, and other substances
- Highly specific in quantification by location in tissue
QWBA Advantages:
- Highly precise quantification capabilities, even at low and high limits of detection
- Spatial resolution at the cellular level
- Gold standard technique for the late-stage regulatory distribution studies
Even though QWBA remains the gold standard technique during late stage for regulatory agencies, QMSI is frequently used in support of the autoradiography in certain circumstances.
Further evaluate the benefits and risks associated with each technology.