Multiplex Immunohistochemistry (mlHC) is an advanced technique that can detect tissue-based biomarkers simultaneously, which transforms the traditional approach of immunohistochemistry. mlHC enables precision medicine in both research and clinical practice because it evaluates various proteins and their spatial distribution within single tissue sections at a cellular level.  

Specific requirements are crucial for achieving high-quality staining and analysis, and to deem a mlHC assay as validated, it must be proven to be analytically reproducible.

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