The next evolution of spatial biology is the transition from exploratory imaging toward controlled generation of quantitative spatial metrics associated with pharmacodynamic-relevant tumor biology.
In non-small cell lung cancer (NSCLC) immuno-oncology studies, the challenge is not simply identifying immune cells within the tissue, but understanding whether their spatial organization reflects biologically meaningful mechanisms associated with therapeutic activity, immune exclusion, suppression, or cytotoxic engagement.
This case study illustrates how a qualified multiplex spatial panel can be deployed on FFPE NSCLC tissue to generate reproducible and biologically interpretable spatial metrics associated with pharmacodynamic (PD)-relevant tumor–immune biology. The objective: implementation of a controlled multiplex framework capable of moving beyond descriptive multiplex imaging toward more quantitative spatial characterization of immune biology.
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