Bile acids are commonly extracted from complex biological samples with methanol -based extraction procedures. However, with these simple extraction procedures, numerous reports have found that accumulating lipids on the analytical column interferes with LC/MS analyses of bile acids. In our experience, these adverse effects included increasing column backpressure, retention time drift and peak shape broadening over multiple injections. In lieu of employing an alternative offline solid -phase extraction (SPE) cleanup, we investigated several column chemistries and mobile phase combinations in conjunction with the simple protein precipitation extraction.
Download the poster, presented at ASMS 2026, to learn how Aliri approached the quantitation of 20 bile acids in human serum and urine.