In clinical studies, 4β-hydroxycholesterol is an important biomarker for assessing the activity of CYP3A. Numerous quantitative methods have been reported for analyzing the total amount of 4β-hydroxycholesterol (free and conjugated) after saponification and derivatization. Derivatization is often required to achieve clinically relevant assay sensitivity when utilizing LCMS for detection, but this additional step can be complex and time-consuming.

Download our poster to learn more.

Download Now