Quantitative Mass Spectrometry Imaging (QMSI) is used to evaluate the amount of a large molecule (higher than 3000 Da)
within tissue. Methodology of quantification using a «pseudo internal standard» covering the sample is explained (“Modified
Standard” Approach) and applied to the example of mouse insulin assessment in pancreas tissue. To ensure fast data
treatment QuantinetixTM software is used in order to calculate the amount of target molecule.
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