This application note focuses on an efficient analysis method which enables the quantification of molecules at the single-cell level. Accurate RNA quantification in single molecules is crucial to understand the dynamics of gene expression and regulation. 
In this study, individual molecules were imaged in fixed cells and DNA staining was performed in tumoral areas of the tissue. Analysis results showcase quantification of single cells and provide an understanding of cell-to-cell interactions in cancer cells and other molecules.  
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